Journal: American Journal of Cancer Research

Article Title: Tolypothrix Dichloromethane Ethylacetate fraction (TDEF) inhibits cisplatin resistance H357 cell through PI3K/AKT/beta-catenin pathway

doi: 10.62347/JTNQ4812

Figure Lengend Snippet: A. Oral cancer cell line H357 sensitive and H357cisR was seeded on 6 well plates and treated with different concentrations 5, 10, and 15 ug mL-1. With respect to time, the cell morphology changes and becomes rounded in shape, and at higher concentration cell starts to detach from the surface. B. H357CisR cells were treated with indicated dose of TDEF for 48 hrs after which cell death was determined by annexin V/7AAD assay using flow cytometer. The dot plots represent the percentage of early apoptotic (lower right) and late apoptotic cells (upper right). C. Bar diagrams indicate the percentage of cell death; black color portion indicates percentage of early apoptosis and grey color indicates percentage of late apoptosis occur with respective treated groups. D. TDEF treated H357cisR cell pellet was fixed with 70% ethanol and stained with propidium iodide and the cell distribution in different cell cycle phases was analyzed by FACS. Cell cycle arrest was shown highest at 10 ug mL-1 but the cell with the higher concentration of extract has experience apoptosis and necrosis. The percentage of the G1 population was found to be 65.1%, and the control G1 cell population was 47.8%.

Article Snippet: Protein array analysis Human apoptosis array analysis was performed using a proteome profiler apoptotic array kit (ARY009; R & D system.

Techniques: Concentration Assay, Flow Cytometry, Staining, Control

Journal: American Journal of Cancer Research

Article Title: Tolypothrix Dichloromethane Ethylacetate fraction (TDEF) inhibits cisplatin resistance H357 cell through PI3K/AKT/beta-catenin pathway

doi: 10.62347/JTNQ4812

Figure Lengend Snippet: A. The Pp53 and P21 signalling pathways are strongly activated by TDEF treatment. The human apoptosis proteome profiler array used a protein extract (400 µg). The intensities of each array spot were visualized and further quantified using an image. B. The graph shows the relative fold change of proteins with significant differences upon TDEF treatment, setting 1 for control (no treatment of TDEF). Protein levels with higher than ± 2 folds are considered candidates for TDEF-induced cell death.

Article Snippet: Protein array analysis Human apoptosis array analysis was performed using a proteome profiler apoptotic array kit (ARY009; R & D system.

Techniques: Control

Journal: Experimental and Therapeutic Medicine

Article Title: Insulin-like growth factor-binding proteins play a significant role in the molecular response to imatinib in chronic myeloid leukemia patients

doi: 10.3892/etm.2019.8364

Figure Lengend Snippet: Results of apoptosis antibody array data. (A) Heat maps of differentially expressed proteins between K562 and K562/G cells, the cut-off value was set as follows: Fold-change <1.2 or <0.83, fluorescent value >150 and P<0.05 (K562/G VS K562). (B) Kyoto Encyclopedia of Genes and Genomes pathway analysis of differentially expressed proteins.

Article Snippet: An apoptosis antibody array (Ray Biotech, Inc.) was used to explore the differences in the apoptotic protein profile between K562 and K562/G cells.

Techniques: Ab Array

Journal: International Journal of Molecular Sciences

Article Title: Association of SNPs in the PAI1 Gene with Disease Recurrence and Clinical Outcome in Bladder Cancer

doi: 10.3390/ijms24054943

Figure Lengend Snippet: PAI1 rs7242 is involved in the resistance to apoptosis induced by cisplatin. ( A ) Protein and mRNA expression of PAI1 clones in stable transfectants in UROtsa and 5637 cell lines. ( B ) Apoptosis assays using RealTime-Glo™ Annexin V Apoptosis and Caspase-Glo ® 3/7 Assays. ( C ) Profiling the expression of multiple proteins associated with apoptosis induced by cisplatin using a membrane-based sandwich immunoassay. ( D ) mRNA expression levels of ATR-CLSPN-CHEK1 pathway. ( E ) Silencing CLSPN by siRNA in parental UROtsa and 5637 cell lines. ( F ) Cell survival assay after cisplatin treatment. ( G ) Apoptosis assay after cisplatin treatment. Bars represent SE. ** p < 0.01; *** p < 0.001, **** p < 0. 0001.

Article Snippet: To investigate how PAI1 might inhibit apoptosis, we used an array (Human Apoptosis Array Kit; ARY 009, Promega, Madison, WI, USA) which monitors 35 apoptosis-related proteins.

Techniques: Expressing, Clone Assay, Membrane, Clonogenic Cell Survival Assay, Apoptosis Assay

Journal: BMC Neuroscience

Article Title: Neuronal hypoxia in vitro : Investigation of therapeutic principles of HUCB-MNC and CD133 + stem cells

doi: 10.1186/1471-2202-9-91

Figure Lengend Snippet: Time course of apoptosis and necrosis of post-hypoxic neuronal cells in direct co-cultures with tMNC, CD133 + and CD133 - . Box plots showing time course of apoptosis (A) and necrosis/late apoptosis (B) in direct co-cultures of post-hypoxic neuronal cells with tMNC, CD133 - or CD133 + (colored plots). Grey boxes display the development of cell death in post-hypoxic neuronal cells directly after hypoxic pre-cultivation of SH-SY5Y cells (Day 0) and 1–3 days post-hypoxia. The ordinate represents the percentage of apoptotic and necrotic cells, respectively. Rates of apoptosis and necrosis were calculated in relation to the total number of SH-SY5Y cells.

Article Snippet: Cytometric Bead Array for human apoptosis (CBA, Becton Dickinson, Erembodegem, Belgium) was used to quantify the apoptosis specific parameter cleaved Poly-ADP-Ribose-Polymerase I (PARP) in lysates of post-hypoxic neuronal cells after indirect co-cultivation with tMNC and CD133 - .

Techniques:

Journal: BMC Neuroscience

Article Title: Neuronal hypoxia in vitro : Investigation of therapeutic principles of HUCB-MNC and CD133 + stem cells

doi: 10.1186/1471-2202-9-91

Figure Lengend Snippet: Effects of direct and indirect application of tMNC and CD133 - on apoptosis and necrosis of post-hypoxic neuronal cells . Box plots represent the percentage of annexin-V positive post-hypoxic neuronal cells (A, B) and of PI positive post-hypoxic neuronal cells (C, D) in direct and indirect co-cultures. Co-cultures were performed with tMNC (A, C) and CD133 - (B, D). The extents of apoptosis (annexin-V-binding) and necrosis (PI) were analyzed for three days after hypoxia.

Article Snippet: Cytometric Bead Array for human apoptosis (CBA, Becton Dickinson, Erembodegem, Belgium) was used to quantify the apoptosis specific parameter cleaved Poly-ADP-Ribose-Polymerase I (PARP) in lysates of post-hypoxic neuronal cells after indirect co-cultivation with tMNC and CD133 - .

Techniques: Binding Assay

Journal: BMC Neuroscience

Article Title: Neuronal hypoxia in vitro : Investigation of therapeutic principles of HUCB-MNC and CD133 + stem cells

doi: 10.1186/1471-2202-9-91

Figure Lengend Snippet: Direct comparison of effects of tMNC and CD133 - on late apoptosis (PARP cleavage) . Bar chart showing concentrations of cleaved PARP in lysates of post-hypoxic neuronal cells which were indirectly co-cultivated with tMNC (grey shaded bars) or CD133 - (black bars). Controls are post-hypoxic mono-cultures (grey bars) and normoxic cultures (white bars). Each bar visualizes pooled samples obtained from three independent experiments.

Article Snippet: Cytometric Bead Array for human apoptosis (CBA, Becton Dickinson, Erembodegem, Belgium) was used to quantify the apoptosis specific parameter cleaved Poly-ADP-Ribose-Polymerase I (PARP) in lysates of post-hypoxic neuronal cells after indirect co-cultivation with tMNC and CD133 - .

Techniques: Comparison